Guanxing Chen

Nov 6, 2024 | digitalcommons.library.tmc.edu | Guanxing Chen |Qin Li |Timothy Webb |Jiusheng Yan

Publication Date6-5-2023JournalThe Journal of General PhysiologyAbstractThe large-conductance, Ca2+-, and voltage-activated K+ (BK) channel consists of the pore-forming α (BKα) subunit and regulatory β and γ subunits. The γ1-3 subunits facilitate BK channel activation by shifting the voltage-dependence of channel activation toward the hyperpolarization direction by about 50-150 mV in the absence of Ca2+.

Oct 18, 2024 | nature.com | Guanxing Chen |Jianhua Yao |Linlin You |Calvin Yu-Chian Chen |Zhenchao Tang |Shouzhi Chen

Heterogeneous feature spaces and technical noise hinder the cellular data integration and imputation. The high cost of obtaining matched data across modalities further restricts analysis. Thus, there’s a critical need for deep learning approaches to effectively integrate and impute unpaired multi-modality single-cell data, enabling deeper insights into cellular behaviors. To address these issues, we introduce the Modal-Nexus Auto-Encoder (Monae). Leveraging regulatory relationships between modalities and employing contrastive learning within modality-specific auto-encoders, Monae enhances cell representations in the unified space. The integration capability of Monae furnishes it with modality-complementary cellular representations, enabling the generation of precise intra-modal and cross-modal imputation counts for extensive and complex downstream tasks. In addition, we develop Monae-E (Monae-Extension), a variant of Monae that can converge rapidly and support biological discoveries. Evaluations on various datasets have validated Monae and Monae-E’s accuracy and robustness in multi-modality cellular data integration and imputation. Heterogeneous feature spaces and technical noise hinder the cellular data integration and further analysis. Here, authors report a Modal-Nexus Auto-Encoder (Monae) to effectively integrate unpaired multi-modality cellular data and generate imputation counts for downstream analysis.

Apr 17, 2024 | cell.com | Qin Li |Guanxing Chen |Jiusheng Yan

Abstract Voltage-gated potassium channels are critical in modulating cellular excitability, with Slo (slowpoke) channels forming a unique family characterized by their large conductance and dual regulation by electrical signals and intracellular messengers. Despite their structural and evolutionary similarities, Slo1 and Slo3 channels exhibit significant differences in their voltage-gating properties.

Jun 28, 2023 | biorxiv.org | Guanxing Chen |Qin Li |Jiusheng Yan

New Results doi: https://doi.org/10.1101/2023.06.26.546634 AbstractThe large-conductance, Ca2+-activated K+ (BK) channels consist of the Ca2+- and voltage-sensing and pore-forming α subunits and regulatory auxiliary β or γ subunits. Concatenated tandem constructs have been proved to be necessary and powerful in understanding the subunit stoichiometry of K+ channel gating and regulation by constraining the stoichiometry, organization, and mutation of individual subunits.