Haneen Sohail Alsehli

Jul 3, 2024 | cell.com | Dylan Stavish |Christopher Price |Gabriele Gelezauskaite |Haneen Sohail Alsehli

Highlights•Increase in chromosome 1q gains in recent years is linked to feeder-free cultures•Feeder-free conditions (E8/vitronectin) confer high levels of genome damage on hPSC•Amplification of MDM4 on chromosome 1q alleviates genome damage-induced apoptosis•Karyotypically abnormal cells have a context-dependent selective advantageSummaryCulture-acquired variants in human pluripotent stem cells (hPSCs) hinder their applications in research and clinic.