Gabriele Gelezauskaite

Jul 3, 2024 | cell.com | Dylan Stavish |Christopher Price |Gabriele Gelezauskaite |Haneen Sohail Alsehli

Highlights•Increase in chromosome 1q gains in recent years is linked to feeder-free cultures•Feeder-free conditions (E8/vitronectin) confer high levels of genome damage on hPSC•Amplification of MDM4 on chromosome 1q alleviates genome damage-induced apoptosis•Karyotypically abnormal cells have a context-dependent selective advantageSummaryCulture-acquired variants in human pluripotent stem cells (hPSCs) hinder their applications in research and clinic.

Sep 21, 2023 | biorxiv.org | Dylan Stavish |Christopher Price |Gabriele Gelezauskaite |Kimberly A. Leonhard

AbstractSince the first derivation of human pluripotent stem cells (hPSCs), the number of culture conditions has steadily increased, making hPSC culture more facile. Nonetheless, there remains the persistent issue of culture-acquired genetic changes, hampering the reproducibility of hPSC research and jeopardising their clinical use.