Hasindu Gamaarachchi

Aug 13, 2024 | academic.oup.com | Hasindu Gamaarachchi

Nanopore sequencing enables direct analysis of native DNA, RNA, and protein molecules, with countless potential applications across the life sciences (Wang et al. 2021). Devices from Oxford Nanopore Technologies (ONT) measure the displacement of ionic current as a DNA or RNA molecule passes through a nanoscale protein pore. The device records time-series current signal data—commonly referred to as ‘squiggle’ data—which can be ‘basecalled’ into sequence reads and/or analysed directly (Wang et al.

Mar 3, 2024 | nature.com | Skye D. Fissette |Deniz Kaya |Jillian Hammond |Hasindu Gamaarachchi |Ira W. Deveson |Robert Klose | +2 more

AbstractDNA methylation (5mC) is a repressive gene regulatory mark widespread in vertebrate genomes, yet the developmental dynamics in which 5mC patterns are established vary across species. While mammals undergo two rounds of global 5mC erasure, teleosts, for example, exhibit localized maternal-to-paternal 5mC remodeling.