Hope A Townsend

Nov 23, 2024 | biorxiv.org | Jacob Stanley |Hope A Townsend |Rutendo F. Sigauke |Mary Allen

AbstractTranscription by RNA polymerases is an exquisitely regulated step of the central dogma. Transcription is the primary determinant of cell-state, and most cellular perturbations impact transcription by altering polymerase activity. Thus, detecting changes in polymerase activity yields insight into most cellular processes. Nascent run-on sequencing provides a direct readout of polymerase activity, but no tools exist to model this activity at genes.

Jun 20, 2024 | biorxiv.org | Kaylee Rosenberger |Lauren A. Vanderlinden |Jun Inamo |Hope A Townsend

AbstractBackground: Understanding genetic underpinnings of immune-mediated inflammatory diseases is crucial to improve treatments. Single-cell RNA sequencing (scRNA-seq) identifies cell states expanded in disease, but often overlooks genetic causality due to cost and small genotyping cohorts. Conversely, large genome-wide association studies (GWAS) are commonly accessible.