Ignacio Vázquez-García

May 13, 2024 | nature.com | Alexander N Gorelick |Ignacio Vázquez-García |Marc Williams |Adam C. Weiner |Tyler Funnell |Tricia Park | +5 more

AbstractThe extent of cell-to-cell variation in tumor mitochondrial DNA (mtDNA) copy number and genotype, and the phenotypic and evolutionary consequences of such variation, are poorly characterized. Here we use amplification-free single-cell whole-genome sequencing (Direct Library Prep (DLP+)) to simultaneously assay mtDNA copy number and nuclear DNA (nuDNA) in 72,275 single cells derived from immortalized cell lines, patient-derived xenografts and primary human tumors.