Marc Williams

Sep 30, 2024 | nature.com | Adam C. Weiner |Marc Williams |Nicole Rusk |Samuel Aparicio |Sohrab P. Shah

AbstractDysregulated DNA replication is a cause and a consequence of aneuploidy in cancer, yet the interplay between copy number alterations (CNAs), replication timing (RT) and cell cycle dynamics remain understudied in aneuploid tumors. We developed a probabilistic method, PERT, for simultaneous inference of cell-specific replication and copy number states from single-cell whole genome sequencing (scWGS) data.

May 13, 2024 | nature.com | Alexander N Gorelick |Ignacio Vázquez-García |Marc Williams |Adam C. Weiner |Tyler Funnell |Tricia Park | +5 more

AbstractThe extent of cell-to-cell variation in tumor mitochondrial DNA (mtDNA) copy number and genotype, and the phenotypic and evolutionary consequences of such variation, are poorly characterized. Here we use amplification-free single-cell whole-genome sequencing (Direct Library Prep (DLP+)) to simultaneously assay mtDNA copy number and nuclear DNA (nuDNA) in 72,275 single cells derived from immortalized cell lines, patient-derived xenografts and primary human tumors.

Feb 15, 2023 | securityboulevard.com | Marc Williams

The challenges enterprise IT security teams face today are nothing like they were five years ago. Today enterprises face globally distributed networks of bad actors that wield increasingly sophisticated technological and social tools, designed to infiltrate secure systems and exploit identity-based vulnerabilities.