Jennifer M Umhoefer

Jan 14, 2025 | biorxiv.org | Jennifer M Umhoefer |Maya M. Arce |Sean Whalen |Rama Dajani

AbstractFOXP3 is a lineage–defining transcription factor (TF) for immune–suppressive regulatory T cells (Tregs). While mice exclusively express FOXP3 in Tregs, humans also transiently express FOXP3 in stimulated conventional CD4+ T cells (Tconvs). Mechanisms governing these distinct expression patterns remain unknown. Here, we performed CRISPR screens tiling the FOXP3 locus and targeting TFs in human Tregs and Tconvs to discover cis–regulatory elements (CREs) and trans–regulators of FOXP3.

Sep 2, 2024 | biorxiv.org | Maya M. Arce |Rama Dajani |Julia A. Belk |Jennifer M Umhoefer

AbstractFOXP3 is a lineage–defining transcription factor that controls differentiation and maintenance of suppressive function of regulatory T cells (Tregs). Foxp3 is exclusively expressed in Tregs in mice. However, in humans, FOXP3 is not only constitutively expressed in Tregs; it is also transiently expressed in stimulated CD4+CD25– conventional T cells (Tconvs)1–3. Mechanisms governing the expression of FOXP3 in human Tconvs are not understood.