Maya M. Arce

Jan 14, 2025 | biorxiv.org | Jennifer M Umhoefer |Maya M. Arce |Sean Whalen |Rama Dajani

AbstractFOXP3 is a lineage–defining transcription factor (TF) for immune–suppressive regulatory T cells (Tregs). While mice exclusively express FOXP3 in Tregs, humans also transiently express FOXP3 in stimulated conventional CD4+ T cells (Tconvs). Mechanisms governing these distinct expression patterns remain unknown. Here, we performed CRISPR screens tiling the FOXP3 locus and targeting TFs in human Tregs and Tconvs to discover cis–regulatory elements (CREs) and trans–regulators of FOXP3.

Sep 2, 2024 | biorxiv.org | Maya M. Arce |Rama Dajani |Julia A. Belk |Jennifer M Umhoefer

AbstractFOXP3 is a lineage–defining transcription factor that controls differentiation and maintenance of suppressive function of regulatory T cells (Tregs). Foxp3 is exclusively expressed in Tregs in mice. However, in humans, FOXP3 is not only constitutively expressed in Tregs; it is also transiently expressed in stimulated CD4+CD25– conventional T cells (Tconvs)1–3. Mechanisms governing the expression of FOXP3 in human Tconvs are not understood.

May 29, 2024 | nature.com | Jacob W. Freimer |Salvador Casaní-Galdón |Maya M. Arce |Bence Daniel |Katalin Sandor |Ralf Schmidt | +3 more

AbstractCis-regulatory elements (CREs) interact with trans regulators to orchestrate gene expression, but how transcriptional regulation is coordinated in multi-gene loci has not been experimentally defined. We sought to characterize the CREs controlling dynamic expression of the adjacent costimulatory genes CD28, CTLA4 and ICOS, encoding regulators of T cell-mediated immunity.

Sep 17, 2023 | biorxiv.org | Joshua Weinstock |Maya M. Arce |Jacob W. Freimer |Mineto Ota

AbstractThe effects of genetic variation on complex traits act mainly through changes in gene regulation. Although many genetic variants have been linked to target genes in cis, the trans-regulatory cascade mediating their effects remains largely uncharacterized. Mapping trans-regulators based on natural genetic variation, including eQTL mapping, has been challenging due to small effects. Experimental perturbation approaches offer a complementary and powerful approach to mapping trans-regulators.