Jiang-An Yin

Apr 28, 2024 | biorxiv.org | Adriano Aguzzi |Jiang-An Yin |David J. Vocadlo |Kathi Ging

AbstractMutations in the GBA gene, which encodes the lysosomal enzyme β-glucocerebrosidase (GCase), are the most prevalent genetic susceptibility factor for Parkinson's disease (PD). However, only approximately 20% of carriers develop the disease, suggesting the presence of genetic modifiers influencing the risk of developing PD in the presence of GBA mutations.

Feb 5, 2024 | nature.com | Michela Serresi |Ben Jiang |Jiang-An Yin |Adriano Aguzzi |Iros Barozzi

AbstractDescriptive data are rapidly expanding in biomedical research. Instead, functional validation methods with sufficient complexity remain underdeveloped. Transcriptional reporters allow experimental characterization and manipulation of developmental and disease cell states, but their design lacks flexibility.

Jul 20, 2023 | biorxiv.org | Jiang-An Yin |Lukas Frick |Manuel C Scheidmann |Tingting Liu

AbstractArrayed CRISPR libraries extend the scope of gene-perturbation screens but require large numbers of efficacious sgRNA-expressing vectors. Using a newly invented liquid-phase plasmid cloning methodology, we constructed genome-wide arrayed libraries for human gene ablation (19,936 plasmids), activation, and epigenetic silencing (22,442 plasmids). At least 76% of each plasmid preparation encoded an intact array of 4 non-overlapping sgRNAs designed to tolerate most human DNA polymorphisms.

Jun 2, 2023 | frontiersin.org | Jiang-An Yin |Zhaotao Wang |Miren Revuelta

Distribution of astrocytesAstrocytes are the dominant and most diverse cell type in the mammalian central nervous system (CNS), accounting for 20%–40% of all glial cells (Rowitch and Kriegstein, 2010). Astrocytes are named from their star-shaped appearance, with numerous long and branched processes radiating from their cell bodies to occupy the interstitial spaces between neuronal cells, thereby supporting and separating neurons.