Iros Barozzi

Oct 9, 2024 | nature.com | Samuel Abassah-Oppong |Raquel Rouco |Cailyn H. Spurrell |Fabrice Darbellay |Jennifer A. Akiyama |Javier Lopez-Rios | +5 more

AbstractApproximately a quarter of the human genome consists of gene deserts, large regions devoid of genes often located adjacent to developmental genes and thought to contribute to their regulation. However, defining the regulatory functions embedded within these deserts is challenging due to their large size.

Mar 20, 2024 | nature.com | Zhuoxin Chen |Valentina Snetkova |Benjamin Clock |Iros Barozzi |Brandon J. Mannion |Ana Alcaina-Caro | +3 more

AbstractRemote enhancers are thought to interact with their target promoters via physical proximity, yet the importance of this proximity for enhancer function remains unclear. Here we investigate the three-dimensional (3D) conformation of enhancers during mammalian development by generating high-resolution tissue-resolved contact maps for nearly a thousand enhancers with characterized in vivo activities in ten murine embryonic tissues.

Mar 5, 2024 | nature.com | Sudha Rajderkar |Maria Amaral |Laura Cook |Fabrice Darbellay |Cailyn H. Spurrell |Marco Osterwalder | +9 more

AbstractThe genetic basis of human facial variation and craniofacial birth defects remains poorly understood. Distant-acting transcriptional enhancers control the fine-tuned spatiotemporal expression of genes during critical stages of craniofacial development. However, a lack of accurate maps of the genomic locations and cell type-resolved activities of craniofacial enhancers prevents their systematic exploration in human genetics studies.

Feb 5, 2024 | nature.com | Michela Serresi |Ben Jiang |Jiang-An Yin |Adriano Aguzzi |Iros Barozzi

AbstractDescriptive data are rapidly expanding in biomedical research. Instead, functional validation methods with sufficient complexity remain underdeveloped. Transcriptional reporters allow experimental characterization and manipulation of developmental and disease cell states, but their design lacks flexibility.

Jul 6, 2023 | nature.com | Iros Barozzi |Marco Osterwalder |Susan Mackem |Terence D. Capellini |Diane E. Dickel |Nicoletta Bobola | +5 more

AbstractA lingering question in developmental biology has centered on how transcription factors with widespread distribution in vertebrate embryos can perform tissue-specific functions. Here, using the murine hindlimb as a model, we investigate the elusive mechanisms whereby PBX TALE homeoproteins, viewed primarily as HOX cofactors, attain context-specific developmental roles despite ubiquitous presence in the embryo.