Jinfeng Shang

Nov 6, 2024 | cell.com | Sumin Feng |Kaiwen Ivy Liu |Jinfeng Shang |Lisa Hoeg |William Yang |Guillermo Sastre-Moreno | +6 more

Keywords synthetic lethality DNA replication DNA replication fork ATP translocase genome stability simple repeats DNA double-strand breaks Introduction The human genome encodes 33 SNF2-family ATP-dependent translocases.1,2 While this family is generally viewed as consisting of chromatin remodeling enzymes, at least 11 of the 33 SNF2-family proteins (CHD1L, RAD54B, RAD54L, RAD54L2, HLTF, SHPRH, ERCC6, ERCC6L/PICH, ERCC6L2, SMARCAL1, and ZRANB3) are directly implicated in the maintenance of...

Feb 27, 2024 | biorxiv.org | Sumin Feng |Kaiwen Ivy Liu |Lisa Hoeg |Jinfeng Shang

AbstractDNA replication stress is a threat to genome integrity. The large SNF2-family of ATPases participates in preventing and mitigating DNA replication stress by employing their ATP-driven motor to remodel DNA or DNA-bound proteins. To understand the contribution of these ATPases in genome maintenance, we undertook CRISPR-based synthetic lethality screens with three SNF2-type ATPases: SMARCAL1, ZRANB3 and HLTF.