jingyi Cai

Oct 16, 2024 | onlinelibrary.wiley.com | fan wei |jingyi Cai |Yufeng Mao |Ruoyu Wang

1 Introduction Microbial cell factories with efficient biosynthetic pathways enable the production of various chemicals from renewable carbon sources.[1] Over the past decades, these cell factories have successfully produced a diverse range of chemical compounds,[2] including biofuels,[3] platform chemicals,[4] pharmaceuticals,[5] and food additives.[6] Pathway yield (YP) is the amount of a product formed from a substrate, computed based on the stoichiometry of the host.[7] YP is a crucial...

Jun 10, 2023 | onlinelibrary.wiley.com | jingyi Cai |Xiaoping Liao |Haihe Laboratory |Yufeng Mao

Recent advances in biofoundries have enabled the construction of a large quantity of strains in parallel, accelerating the Design-Build-Test-Learn (DBTL) cycles for strain development. However, the construction of a large number of strains by iterative gene manipulation is still time-consuming and costly, posing a challenge for the development of commercial strains.