Jochen Dobner

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Reassessment of marker genes in human induced pluripotent stem cells for enhanced quality control - Nature Communications

Oct 1, 2024 | nature.com | Jochen Dobner |Sebastian Diecke |Jean Krutmann |Alessandro Prigione

AbstractHuman induced pluripotent stem cells (iPSCs) have great potential in research, but pluripotency testing faces challenges due to non-standardized methods and ambiguous markers. Here, we use long-read nanopore transcriptome sequencing to discover 172 genes linked to cell states not covered by current guidelines.
Mitochondrial DNA integrity and metabolome profile are preserved in the human induced pluripotent stem cell reference line KOLF2.1J

Feb 22, 2024 | cell.com | Thach G. Nguyen |Andreas Dunkel |Alessandro Prigione |Jochen Dobner

Highlights•The mtDNA of the KOLF2.1J reference line is intact•The metabolomic signature of the KOLF2.1J reference line is inconspicuous•We recommend assessing mtDNA integrity of iPSC lines•ERCC6R683X/R683X Cockayne syndrome model line shows an altered metabolomic signatureSummaryQuality control of human induced pluripotent stem cells (iPSCs) is critical to ensure reproducibility of research.

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