Alessandro Prigione

Oct 1, 2024 | nature.com | Jochen Dobner |Sebastian Diecke |Jean Krutmann |Alessandro Prigione

AbstractHuman induced pluripotent stem cells (iPSCs) have great potential in research, but pluripotency testing faces challenges due to non-standardized methods and ambiguous markers. Here, we use long-read nanopore transcriptome sequencing to discover 172 genes linked to cell states not covered by current guidelines.

Mar 22, 2024 | cell.com | Stephanie Le |Heinrich Heine |Carmen Menacho |Alessandro Prigione

AbstractIn a recent study, Rylaarsdam and colleagues revealed that mutant PACS1 gene, which causes a rare neurodevelopmental syndrome, affects the firing ability of human neurons without dysregulating the cellular architecture of brain organoids. These findings suggest aberrant neuronal electrophysiology as a possible interventional target for pediatric diseases impairing brain development.

Feb 22, 2024 | cell.com | Thach G. Nguyen |Andreas Dunkel |Alessandro Prigione |Jochen Dobner

Highlights•The mtDNA of the KOLF2.1J reference line is intact•The metabolomic signature of the KOLF2.1J reference line is inconspicuous•We recommend assessing mtDNA integrity of iPSC lines•ERCC6R683X/R683X Cockayne syndrome model line shows an altered metabolomic signatureSummaryQuality control of human induced pluripotent stem cells (iPSCs) is critical to ensure reproducibility of research.