Sebastian Diecke

Oct 1, 2024 | nature.com | Jochen Dobner |Sebastian Diecke |Jean Krutmann |Alessandro Prigione

AbstractHuman induced pluripotent stem cells (iPSCs) have great potential in research, but pluripotency testing faces challenges due to non-standardized methods and ambiguous markers. Here, we use long-read nanopore transcriptome sequencing to discover 172 genes linked to cell states not covered by current guidelines.

Aug 22, 2024 | nature.com | Selene Lickfett |Agnieszka Rybak-Wolf |Carmen Menacho |Stephanie Le |Tancredi Massimo Pentimalli |Daniel Oehler | +14 more

AbstractExpansion of the glutamine tract (poly-Q) in the protein huntingtin (HTT) causes the neurodegenerative disorder Huntington’s disease (HD). Emerging evidence suggests that mutant HTT (mHTT) disrupts brain development. To gain mechanistic insights into the neurodevelopmental impact of human mHTT, we engineered male induced pluripotent stem cells to introduce a biallelic or monoallelic mutant 70Q expansion or to remove the poly-Q tract of HTT.

Dec 19, 2023 | nature.com | Benjamin R. Rost |Gary J. Bassell |Wilfried Rossoll |Sebastian Diecke |Séverine Kunz

AbstractThe complex neuromuscular network that controls body movements is the target of severe diseases that result in paralysis and death. Here, we report the development of a robust and efficient self-organizing neuromuscular junction (soNMJ) model from human pluripotent stem cells that can be maintained long-term in simple adherent conditions.

Nov 20, 2023 | insights.bio | Sebastian Diecke |Hannah Johannsen |Parivash Nouri

Join us for an informative webinar on creating induced pluripotent stem cells (iPSCs), which sheds light on the strengths of the mRNA technique compared to other methods.

Oct 12, 2023 | science.org | Kimberly Siletti |Nikolas L. Jorstad |Thomas Chartrand |Filiz Sila Rizalar |Astrid G. Petzoldt |Shuhan Kong | +13 more

Published In ScienceVolume 382 | Issue 666713 October 2023Article versionsSubmission historyReceived: 2 December 2022Accepted: 16 August 2023Published in print: 13 October 2023PermissionsRequest permissions for this article. AcknowledgmentsWe thank the FMP mass spectrometry core facility, especially H. Stephanowitz for proteomic analysis, W.-T. Lo (FMP, Berlin) for assistance with protein purification and liposome binding assays, and B. Van Rossum for help with the artwork. We further thank M.