Johannes Stein

2 weeks ago | pubs.acs.org | Alexander V Hillsley |Johannes Stein |Paul Tillberg |David Stern

1. IntroductionClick to copy section linkSection link copied!Molecular counting aims to determine the absolute number of subunits in a complex, a quantity that is often essential to understanding the underlying biology of a system. (1−6) Within a complex, subunits of interest are often separated by only a few nanometers and thus cannot be quantified through standard fluorescence microscopy techniques.

Feb 5, 2024 | biorxiv.org | Johannes Stein |Maria Ericsson |Michel Nofal |Lorenzo Magni

AbstractDNA-PAINT combined with total Internal Reflection Fluorescence (TIRF) microscopy enables the highest localization precisions, down to single nanometers in thin biological samples, due to TIRF's unique method for optical sectioning and attaining high contrast. However, most cellular targets elude the accessible TIRF range close to the cover glass and thus require alternative imaging conditions, affecting resolution and image quality.