Paul Tillberg

1 month ago | nature.com | Hua Zhang |Lang Ding |Amy Hu |Xudong Shi |Penghsuan Huang |Haiyan Lu | +3 more

The spatial distribution of diverse biomolecules in multicellular organisms is essential for their physiological functions. High-throughput in situ mapping of biomolecules is crucial for both basic and medical research, and requires high scanning speed, spatial resolution, and chemical sensitivity. Here we developed a tissue-expansion method compatible with matrix-assisted laser desorption/ionization mass-spectrometry imaging (TEMI). TEMI reaches single-cell spatial resolution without sacrificing voxel throughput and enables the profiling of hundreds of biomolecules, including lipids, metabolites, peptides (proteins), and N-glycans. Using TEMI, we mapped the spatial distribution of biomolecules across various mammalian tissues and uncovered metabolic heterogeneity in tumors. TEMI can be easily adapted and broadly applied in biological and medical research, to advance spatial multi-omics profiling. Tissue-expansion method compatible with mass-spectrometry imaging (TEMI) enables the profiling of lipids, metabolites, peptides, proteins, and N-glycans in complex tissues with high spatial resolution, advancing spatially resolved multi-omics mapping.

2 months ago | pubs.acs.org | Alexander V Hillsley |Johannes Stein |Paul Tillberg |David Stern

1. IntroductionClick to copy section linkSection link copied!Molecular counting aims to determine the absolute number of subunits in a complex, a quantity that is often essential to understanding the underlying biology of a system. (1−6) Within a complex, subunits of interest are often separated by only a few nanometers and thus cannot be quantified through standard fluorescence microscopy techniques.