Jonathan S. Weissman

Aug 24, 2024 | biorxiv.org | Chen Weng |Jonathan S. Weissman |Vijay Sankaran

AbstractThe detection of mitochondrial DNA (mtDNA) mutations in single cells holds considerable potential to define clonal relationships coupled with information on cell state in humans. Previous methods focused on higher heteroplasmy mutations that are limited in number and can be influenced by functional selection, introducing biases for lineage tracing.

May 12, 2024 | nature.com | Jonathan S. Weissman |Omar O. Abudayyeh

Correction to: Nature Reviews Molecular Cell Biology https://doi.org/10.1038/s41580-023-00697-6, published online 2 February 2024In the version of the article initially published, there were errors in Fig. 1b where the text now reading “Transversion C to G” and “Transition A to G” originally read “Transition C to G” and “Transversions A to G.” The figure has now been amended in the HTML and PDF versions of the article. About this articleVilliger, L., Joung, J., Koblan, L. et al.

Feb 1, 2024 | nature.com | Jonathan S. Weissman |Omar O. Abudayyeh

AbstractOur ability to edit genomes lags behind our capacity to sequence them, but the growing understanding of CRISPR biology and its application to genome, epigenome and transcriptome engineering is narrowing this gap. In this Review, we discuss recent developments of various CRISPR-based systems that can transiently or permanently modify the genome and the transcriptome.