Juliet Nicodemus

1 week ago | jneurosci.org | Juliet Nicodemus |Christine Liu |Valerie Tan |William J. Romanow

Human postmortem brain tissuesFrozen human brain tissue samples from Brodmann area (BA) 8/9 (prefrontal cortex, PFC) and BA 21 (medial temporal gyrus, MTG) were obtained from multiple brain banks and stored at −80°C. Brain bank tissues sources included: Dalhousie, Emory University, Neurobiobank (Sepulveda), Southwest Dementia Brain Bank, University of California San Diego ADRC, and Washington University.

1 month ago | jneurosci.org | Christine Liu |Valerie Tan |William J. Romanow |Juliet Nicodemus

AbstractReverse transcriptase (RT) activity in the human brain has been inferred through somatic retroinsertion/retrotransposition events, however actual endogenous enzymatic activities and sources remain unclear. L1 (LINE-1) retrotransposons bicistronically express ORF2, containing RT and endonuclease (EN) domains, and RNA binding protein ORF1, together enabling L1 retrotransposition and contributing to somatic genomic mosaicism (SGM).