William J. Romanow

1 week ago | jneurosci.org | Juliet Nicodemus |Christine Liu |Valerie Tan |William J. Romanow

Human postmortem brain tissuesFrozen human brain tissue samples from Brodmann area (BA) 8/9 (prefrontal cortex, PFC) and BA 21 (medial temporal gyrus, MTG) were obtained from multiple brain banks and stored at −80°C. Brain bank tissues sources included: Dalhousie, Emory University, Neurobiobank (Sepulveda), Southwest Dementia Brain Bank, University of California San Diego ADRC, and Washington University.

1 month ago | jneurosci.org | Christine Liu |Valerie Tan |William J. Romanow |Juliet Nicodemus

AbstractReverse transcriptase (RT) activity in the human brain has been inferred through somatic retroinsertion/retrotransposition events, however actual endogenous enzymatic activities and sources remain unclear. L1 (LINE-1) retrotransposons bicistronically express ORF2, containing RT and endonuclease (EN) domains, and RNA binding protein ORF1, together enabling L1 retrotransposition and contributing to somatic genomic mosaicism (SGM).

Dec 1, 2024 | eneuro.org | Christine Liu |Chris Park |Tony Ngo |William J. Romanow

Human brain tissueControl selection was based on the absence of neuropathology relevant to any of the three diseases and RNA integrity number (RIN) ≥ 6. Disease cases were chosen based on neuropathological confirmation of disease and RIN ≥ 6. Controls: 2 male, 3 female; AD: 4 male, 2 female; PD: 4 male, 3 female; DLB: 4 male, 3 female. Fresh frozen brain tissues from the prefrontal cortex (BA 8/9) were obtained from multiple brain banks (Extended Data Table 1-1) and stored at −80°C.