Junhua Niu

Nov 15, 2024 | biorxiv.org | Haicheng Li |Junhua Niu |Yalan Sheng |Yifan Liu

AbstractDNA modifications, such as N6-methyladenine (6mA), play important roles in various processes in eukaryotes. Single molecule, real-time (SMRT) sequencing enables the direct detection of DNA modifications without requiring special sample preparation. However, most SMRT-based studies of 6mA rely on ensemble-level consensus by combining multiple reads covering the same genomic position, which misses the single-molecule heterogeneity.