Yalan Sheng

Nov 15, 2024 | biorxiv.org | Haicheng Li |Junhua Niu |Yalan Sheng |Yifan Liu

AbstractDNA modifications, such as N6-methyladenine (6mA), play important roles in various processes in eukaryotes. Single molecule, real-time (SMRT) sequencing enables the direct detection of DNA modifications without requiring special sample preparation. However, most SMRT-based studies of 6mA rely on ensemble-level consensus by combining multiple reads covering the same genomic position, which misses the single-molecule heterogeneity.

Feb 1, 2024 | biorxiv.org | Xiao Chen |Yalan Sheng |Lili Duan |Aili Ju

AbstractThe ciliate Tetrahymena thermophila is a well-established unicellular model eukaryote, contributing significantly to foundational biological discoveries. Despite its acknowledged importance, current Tetrahymena biology studies face challenges due to gene annotation inaccuracy, particularly the notable absence of untranslated regions (UTRs). To comprehensively annotate the Tetrahymena macronuclear genome, we collected extensive transcriptomic data spanning various cell stages.