Karolina Michalska

Jul 20, 2024 | nature.com | Yasuo Yoshikuni |Tobias J Erb |Ramon Gonzalez |Karolina Michalska |Natalia Maltseva |Philipp Wichmann | +1 more

Abstract2-Hydroxyacyl-CoA lyase/synthase (HACL/S) is a thiamine diphosphate (ThDP)-dependent versatile enzyme originally discovered in the mammalian α-oxidation pathway. HACL/S natively cleaves 2-hydroxyacyl-CoAs and, in its reverse direction, condenses formyl-CoA with aldehydes or ketones. The one-carbon elongation biochemistry based on HACL/S has enabled the use of molecules derived from greenhouse gases as biomanufacturing feedstocks.