Kung-Chi Kao

Sep 20, 2023 | nature.com | Noelia Maldonado-Pérez |Alessio Bevilacqua |Jhan-Jie Peng |Anouk Lepez |Kung-Chi Kao |Francisco Martin | +4 more

AbstractProtective immunity against pathogens or cancer is mediated by the activation and clonal expansion of antigen-specific naive T cells into effector T cells. To sustain their rapid proliferation and effector functions, naive T cells switch their quiescent metabolism to an anabolic metabolism through increased levels of aerobic glycolysis, but also through mitochondrial metabolism and oxidative phosphorylation, generating energy and signalling molecules1,2,3.

Sep 19, 2023 | biocompare.com | Kung-Chi Kao

What do these ratings mean? What do these ratings mean? To titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mice were stained with: PerCP-Cy5.5 anti-CD4 Ab and BV711 anti-CXCR5 Ab in the presence of Zombie NIR in 100 µL PBS at 4C for 30 min.

Sep 15, 2023 | biocompare.com | Kung-Chi Kao

Product Name: T-bet Monoclonal Antibody (eBio4B10 (4B10)), PE-Cyanine7, eBioscience Catalog Number: 25-5825-82To titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mice were stained with FITC anti-CD161 Ab and Zombie NIR in 100 µL PBS at 4C for 30 min. Subsequently, the cells were fixed/permed using eBioscience™ Foxp3 / TF Staining Buffer Set according to the manufacturer's instruction, then stained with PE-Cy7 anti-T-bet Ab in 100 µL Perm buffer at 4C for 30 min.

Sep 15, 2023 | biocompare.com | Kung-Chi Kao

To titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mouse were stained with: PerCP-Cy5.5 anti-CD4 Ab and PB anti-CD103 Ab in the presence of Zombie NIR in 100 μL PBS at 4 degrees C for 30 min.