Lee Dolat

Jun 5, 2024 | biorxiv.org | Chloé I. Charendoff |Yongzheng Wu |Lee Dolat |Felix V Louchez

AbstractChlamydia trachomatis, an intracellular bacterium, highjacks metabolites from the host cell. We provide evidence of global hypermethylation of the host proteome, including histones, during the late stages of infection and that histone hypermethylation is the result of metabolic imbalance favoring the activity of lysine methyl transferases over demethylases. We find that histones hypermethylated at residues H3K4 and H3K9 are distributed throughout the chromatin.

Feb 15, 2024 | elifesciences.org | Robert J. Bastidas |Robert Davidson |Stephen Walsh |Lee Dolat

eLife assessment This important study combines state-of-the art proteomics and genetic manipulation of Chlamydia trachomatis to study the function of a chlamydial effector, Cdu1, with deubiquitination and acetylation activities.

Oct 30, 2023 | biorxiv.org | Robert J. Bastidas |Robert Davidson |Stephen Walsh |Lee Dolat

AbstractMany cellular processes are regulated by ubiquitin-mediated proteasomal degradation. Pathogens can regulate eukaryotic proteolysis through the delivery of proteins with de-ubiquitinating (DUB) activities. The obligate intracellular pathogen Chlamydia trachomatis secretes Cdu1 (ChlaDUB1), a dual deubiquitinase and Lys-acetyltransferase, that promotes Golgi remodeling and survival of infected host cells presumably by regulating the ubiquitination of host and bacterial proteins.

Feb 28, 2023 | biorxiv.org | Robert J. Bastidas |Lee Dolat |Barbara S. Sixt |Jonathan N. Pruneda

Thank you for your interest in spreading the word about bioRxiv. NOTE: Your email address is requested solely to identify you as the sender of this article. Your Email * Your Name * Send To * Enter multiple addresses on separate lines or separate them with commas. Message Subject (Your Name) has forwarded a page to you from bioRxiv Message Body (Your Name) thought you would like to see this page from the bioRxiv website.