Leo James

Apr 3, 2024 | biorxiv.org | Christopher Green |Konstanze F. Winklhofer |Adam Fletcher |Leo James

AbstractAntibody-dependent intracellular neutralisation (ADIN) promotes the rapid proteasomal degradation of viruses and other large substrates in the cytosol. It is dependent on detection of intracellular virus-bound antibodies by the Fc receptor and E3 ligase TRIM21, followed by disassembly of the virus by the unfoldase VCP/p97. It is not known how VCP is recruited to TRIM21. We performed a limited siRNA knock- down screen of known VCP adaptors to determine their involvement in ADIN.