Lili Duan

Feb 6, 2024 | biorxiv.org | Lili Duan |Haicheng Li |Aili Ju |Zhe Zhang

AbstractDNA N6-adenine methylation (6mA) is involved in gene transcription as a potential epigenetic mark in eukaryotes. Despite the reported methyltransferase (MTase) for 6mA methylation in several eukaryotes, the regulatory mechanisms that govern the activity of 6mA MTase remain elusive. Here, we exploited the 6mA MTase AMT1 to elucidate its self-regulation in the unicellular eukaryote Tetrahymena thermophila.

Feb 1, 2024 | biorxiv.org | Xiao Chen |Yalan Sheng |Lili Duan |Aili Ju

AbstractThe ciliate Tetrahymena thermophila is a well-established unicellular model eukaryote, contributing significantly to foundational biological discoveries. Despite its acknowledged importance, current Tetrahymena biology studies face challenges due to gene annotation inaccuracy, particularly the notable absence of untranslated regions (UTRs). To comprehensively annotate the Tetrahymena macronuclear genome, we collected extensive transcriptomic data spanning various cell stages.