Madhav Mani

Sep 23, 2024 | biorxiv.org | Madhav Mani

AbstractSuccessful development from a single cell to a complex, multicellular organism requires that cells differentiate in a coordinated, organized manner, in response to a number of chemical morphogens. While the molecular underpinnings may be extremely complex, the resulting phenomenon, in which a cell decides between one fate or another, is relatively simple.

Mar 15, 2024 | journals.plos.org | Manuel Razo-Mejia |Madhav Mani |Dmitri A. Petrov |Chan Zuckerberg Biohub

Loading metrics Open Access Peer-reviewedResearch Article ? This is an uncorrected proof. Citation: Razo-Mejia M, Mani M, Petrov D (2024) Bayesian inference of relative fitness on high-throughput pooled competition assays. PLoS Comput Biol 20(3): e1011937.

Oct 18, 2023 | biorxiv.org | Madhav Mani |Dmitri A. Petrov |Manuel Razo-Mejia

AbstractThe tracking of lineage frequencies via DNA barcode sequencing enables the quantification of microbial fitness. However, experimental noise coming from biotic and abiotic sources complicates the computation of a reliable inference. We present a Bayesian pipeline to infer relative microbial fitness from high-throughput lineage tracking assays. Our model accounts for multiple sources of noise and propagates uncertainties throughout all parameters in a systematic way.