Michele Simonetti

May 8, 2024 | nature.com | Filip Karlsson |Max Karlsson |Jose Navarro |José Fernández Navarro |Jan Rhomberg-Kauert |Michele Simonetti | +1 more

AbstractThe spatial distribution of cell surface proteins governs vital processes of the immune system such as intercellular communication and mobility. However, fluorescence microscopy has limited scalability in the multiplexing and throughput needed to drive spatial proteomics discoveries at subcellular level.

Apr 23, 2024 | nature.com | Luuk Harbers |Michele Simonetti |Constantin Diekmann |Enrico Berrino |Silvia Giordano |Anna Sapino | +7 more

AbstractSomatic copy number alterations (SCNAs) are pervasive in advanced human cancers, but their prevalence and spatial distribution in early-stage, localized tumors and their surrounding normal tissues are poorly characterized. Here, we perform multi-region, single-cell DNA sequencing to characterize the SCNA landscape across tumor-rich and normal tissue in two male patients with localized prostate cancer.

Nov 11, 2023 | biorxiv.org | Ning Zhang |Luuk Harbers |Michele Simonetti |Constantin Diekmann

AbstractSomatic copy number alterations (SCNAs) are pervasive in advanced human cancers, but their prevalence and spatial distribution in early-stage, localized tumors and their surrounding normal tissues are poorly characterized. Here, we performed multi-region, single-cell DNA sequencing to characterize the SCNA landscape across multiple tumor-rich and normal tissue regions (~125 mm3 tissue cubes) obtained from prostatectomy performed in two patients with localized prostate cancer.