Natalie Aroha Holroyd

Mar 15, 2023 | mdpi.com | Claire Walsh |Lucie Gourmet |Simon Walker-Samuel |Natalie Aroha Holroyd

1. IntroductionEpiscopic microscopy refers to imaging techniques that utilize serial sectioning and block-face imaging to create high-resolution large-volume three-dimensional (3D) datasets. High Resolution Episcopic Microscopy (HREM) and its associated techniques (namely, Optical HREM, Episcopic fluorescent image capture (EFIC) and multi-fluorescent HREM (MF-HREM) (A)), require samples to be embedded in a supporting material to enable automated sectioning at the micron scale.