Nazanin Esmaeili Anvar

Apr 27, 2024 | nature.com | Nazanin Esmaeili Anvar |John G. Doench

AbstractGenetic interactions mediate the emergence of phenotype from genotype, but technologies for combinatorial genetic perturbation in mammalian cells are challenging to scale. Here, we identify background-independent paralog synthetic lethals from previous CRISPR genetic interaction screens, and find that the Cas12a platform provides superior sensitivity and assay replicability.

Sep 5, 2023 | biorxiv.org | Chenchu Lin |Xingdi Ma |Lori Wilson |Nazanin Esmaeili Anvar

AbstractGenetic interactions mediate the emergence of phenotype from genotype, but initial technologies for combinatorial genetic perturbation in mammalian cells suffer from inefficiency and are challenging to scale. Recent focus on paralog synthetic lethality in cancer cells offers an opportunity to evaluate different approaches and improve on the state of the art.