Nicolas Altemose

Sep 5, 2024 | nature.com | Nicolas Altemose |Aaron Straight

AbstractWe recently developed directed methylation with long-read sequencing (DiMeLo-seq) to map protein–DNA interactions genome wide. DiMeLo-seq is capable of mapping multiple interaction sites on single DNA molecules, profiling protein binding in the context of endogenous DNA methylation, identifying haplotype-specific protein–DNA interactions and mapping protein–DNA interactions in repetitive regions of the genome that are difficult to study with short-read methods.

Aug 23, 2023 | nature.com | Arang Rhie |Savannah Hoyt |Dylan Taylor |Nicolas Altemose |Paul W. Hook |Sergey Koren | +37 more

AbstractThe human Y chromosome has been notoriously difficult to sequence and assemble because of its complex repeat structure that includes long palindromes, tandem repeats and segmental duplications1,2,3. As a result, more than half of the Y chromosome is missing from the GRCh38 reference sequence and it remains the last human chromosome to be finished4,5.