Patrick Rabe

Nov 26, 2024 | nature.com | Patrick Rabe |Christopher J Schofield

Abstract2-Oxoglutarate (2OG) dependent Nε-methyl lysine demethylases (JmjC-KDMs) regulate eukaryotic transcription. We report studies showing that isolated forms of all human KDM4 and KDM5 JmjC enzymes catalyse demethylation of N-methylated Arg-3 of histone H2a. Unexpectedly, the results reveal that KDM4E and, less efficiently, KDM4D catalyse C-4 hydroxylation of Arg-20 of H2a on peptides, recombinant H2a, and calf histone extracts, including when the Arg-20 guanidino group is N-methylated.

Sep 17, 2024 | nature.com | Mariska de Munnik |Pauline A. Lang |Patrick Rabe |Jurgen Brem

AbstractThe essential l,d-transpeptidase of Mycobacterium tuberculosis (LdtMt2) catalyses the formation of 3\(\to\)3 cross-links in cell wall peptidoglycan and is a target for development of antituberculosis therapeutics. Efforts to inhibit LdtMt2 have been hampered by lack of knowledge of how it binds its substrate.