Christopher J Schofield

2 months ago | onlinelibrary.wiley.com | Ryan A. Herold |Christopher J Schofield |Fraser A. Armstrong

Supporting Information As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.

Nov 26, 2024 | nature.com | Patrick Rabe |Christopher J Schofield

Abstract2-Oxoglutarate (2OG) dependent Nε-methyl lysine demethylases (JmjC-KDMs) regulate eukaryotic transcription. We report studies showing that isolated forms of all human KDM4 and KDM5 JmjC enzymes catalyse demethylation of N-methylated Arg-3 of histone H2a. Unexpectedly, the results reveal that KDM4E and, less efficiently, KDM4D catalyse C-4 hydroxylation of Arg-20 of H2a on peptides, recombinant H2a, and calf histone extracts, including when the Arg-20 guanidino group is N-methylated.

Aug 29, 2024 | nature.com | Rongling Wang |Amanda Coutts |Rubén García-Martín |Matthias Blüher |Christopher J Schofield |Ioannis Kourtzelis | +8 more

AbstractEnhancing thermogenic brown adipose tissue (BAT) function is a promising therapeutic strategy for metabolic disease. However, predominantly thermoneutral modern human living conditions deactivate BAT. We demonstrate that selective adipocyte deficiency of the oxygen-sensor HIF-prolyl hydroxylase (PHD2) gene overcomes BAT dormancy at thermoneutrality. Adipocyte-PHD2-deficient mice maintain higher energy expenditure having greater BAT thermogenic capacity.

Jun 24, 2024 | nature.com | Lonnie P Swift |Lucy Henderson |Abimael Cruz-Migoni |Christian Renz |Helle D. Ulrich |Joseph A. Newman | +3 more

AbstractDNA double-strand breaks (DSBs), such as those produced by radiation and radiomimetics, are amongst the most toxic forms of cellular damage, in part because they involve extensive oxidative modifications at the break termini. Prior to completion of DSB repair, the chemically modified termini must be removed. Various DNA processing enzymes have been implicated in the processing of these dirty ends, but molecular knowledge of this process is limited.

Jun 13, 2024 | pubs.acs.org | Lennart Brewitz |Christopher J Schofield |R. E. Scott |Charlotte M. N

FiguresReferencesSupport InfoFigure 1 This article references 56 other publications. 1Owen, D. R.; Allerton, C. M. N.; Anderson, A. S.; Aschenbrenner, L.; Avery, M.; Berritt, S.; Boras, B.; Cardin, R. D.; Carlo, A.; Coffman, K. J. An oral SARS-CoV-2 Mpro inhibitor clinical candidate for the treatment of COVID-19.