Priyojit Das

1 month ago | biorxiv.org | Yongwoo Lee |Priyojit Das |Michael Rosenberg |Roy Blum

AbstractUsing CLAP methodology, Guo et al. recently concluded that PRC2 is not an RNA binding protein (RBP). They suggest that prior findings were CLIP artifacts and argue against RNAs direct role in PRC2 regulation. Here, we re-analyze their raw datasets and reach contrary conclusions. Through an independent computational pipeline, we observe significant PRC2 enrichment throughout the transcriptome, including XIST. Applying the authors published computational pipeline also reaffirms PRC2 as an RBP.