Roy Blum

1 month ago | biorxiv.org | Yongwoo Lee |Priyojit Das |Michael Rosenberg |Roy Blum

AbstractUsing CLAP methodology, Guo et al. recently concluded that PRC2 is not an RNA binding protein (RBP). They suggest that prior findings were CLIP artifacts and argue against RNAs direct role in PRC2 regulation. Here, we re-analyze their raw datasets and reach contrary conclusions. Through an independent computational pipeline, we observe significant PRC2 enrichment throughout the transcriptome, including XIST. Applying the authors published computational pipeline also reaffirms PRC2 as an RBP.

Sep 20, 2024 | biorxiv.org | Yongwoo Lee |Roy Blum |Michael Rosenberg |Jeannie Lee

AbstractUsing halo-tagged PRC2 and CLAP methodology, Guo et al. recently came to the conclusion that PRC2 is not an RNA binding protein (RBP). They suggested that previous findings are CLIP artifacts and argue that RNA cannot play a direct role in PRC2 regulation. Here, we perform a re-analysis of the authors raw datasets and come to contrary conclusions. First, CLAP demonstrates significant PRC2 enrichment throughout the transcriptome, including in XIST Repeat A (RepA) motif.