Reyka G. Jayasinghe

2 weeks ago | digitalcommons.library.tmc.edu | Daniel Zhou |Reyka G. Jayasinghe |Siqi Chen |John Herndon

AbstractPancreatic ductal adenocarcinoma is a lethal disease with limited treatment options and poor survival. We studied 83 spatial samples from 31 patients (11 treatment-naïve and 20 treated) using single-cell/nucleus RNA sequencing, bulk-proteogenomics, spatial transcriptomics and cellular imaging. Subpopulations of tumor cells exhibited signatures of proliferation, KRAS signaling, cell stress and epithelial-to-mesenchymal transition.

Oct 30, 2024 | nature.com | Reyka G. Jayasinghe |Michael Iglesia |John Herndon |Austin N. Southard-Smith |Jacqueline L. Mudd |Alla Karpova | +16 more

AbstractTo study the spatial interactions among cancer and non-cancer cells1, we here examined a cohort of 131 tumour sections from 78 cases across 6 cancer types by Visium spatial transcriptomics (ST). This was combined with 48 matched single-nucleus RNA sequencing samples and 22 matched co-detection by indexing (CODEX) samples. To describe tumour structures and habitats, we defined ‘tumour microregions’ as spatially distinct cancer cell clusters separated by stromal components.

Nov 1, 2023 | nature.com | Alla Karpova |Yize Li |Austin N. Southard-Smith |Michael Iglesia |Reyka G. Jayasinghe |Matthew Wyczalkowski | +17 more

AbstractChromatin accessibility is essential in regulating gene expression and cellular identity, and alterations in accessibility have been implicated in driving cancer initiation, progression and metastasis1,2,3,4. Although the genetic contributions to oncogenic transitions have been investigated, epigenetic drivers remain less understood.