Rory Williams

Nov 7, 2024 | science.org | Gordon Rix |Julia Phan |Carsen Stringer |Rory Williams

Editor’s summaryWe can extract intricate design principles underlying protein structure, function, and adaptation from patterns of conservation and change across natural homologs. However, nature took millions of years to generate rich sets of homologous gene sequences, effectively rendering them a nonrenewable resource. Rix et al. developed a high-error-rate orthogonal replication (OrthoRep) system that compresses extensive gene divergence into laboratory time frames at scale.

May 24, 2024 | biorxiv.org | Alexandra M. Paulk |Rory Williams |Chang Liu |UC Irvine

AbstractWe recently developed 'autonomous hypermutation yeast surface display' (AHEAD), a technology that enables the rapid generation of potent and specific antibodies in yeast. AHEAD pairs yeast surface display with an error-prone orthogonal DNA replication system (OrthoRep) to continuously and rapidly mutate surface-displayed antibodies, thereby enabling enrichment for stronger binding variants through repeated rounds of cell growth and fluorescence activated cell sorting (FACS).

Jan 25, 2024 | science.org | Alejandro Couce |Aidan Slattery |Sergey Ivanov |Rory Williams

PerspectiveSynthetic BiologyOrthogonal replication enables rapid continuous biomolecular evolution in Escherichia coliRory L. Williams and Chang C. Liu [email protected] Info & AffiliationsAbstractDirected evolution is a powerful strategy for engineering biomolecules.