Sara Cuylen-Haering

May 23, 2024 | cell.com | Sara Cuylen-Haering

HighlightsA new generation of cell sorters combines cellular imaging with sorting cells based on brightfield or fluorescence microscopy information. Specialized imaging technologies allow for the acquisition of blur-free images of cells in flow.

Nov 6, 2023 | nature.com | Yuki Hayashi |Ievgeniia Zagoriy |Jan Ellenberg |Christoph Müller |Sara Cuylen-Haering

AbstractCryo-electron tomography (cryo-ET) allows for label-free high-resolution imaging of macromolecular assemblies in their native cellular context. However, the localization of macromolecules of interest in tomographic volumes can be challenging. Here we present a ligand-inducible labeling strategy for intracellular proteins based on fluorescent, 25-nm-sized, genetically encoded multimeric particles (GEMs).