
Yuki Hayashi
Articles
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Jan 13, 2025 |
viarail.ca | Yuki Hayashi
Skip hibernation and take the train to Toronto this winter! Canada’s most populous city offers sparkling streetscapes, eclectic restaurants, unique landmarks, plus arts and culture for all ages. Classic Canadiana awaits too, with ice skating, sledding, hiking, and winter festivals.
Genetically encoded multimeric tags for subcellular protein localization in cryo-EM - Nature Methods
Nov 6, 2023 |
nature.com | Yuki Hayashi |Ievgeniia Zagoriy |Jan Ellenberg |Christoph Müller |Sara Cuylen-Haering
AbstractCryo-electron tomography (cryo-ET) allows for label-free high-resolution imaging of macromolecular assemblies in their native cellular context. However, the localization of macromolecules of interest in tomographic volumes can be challenging. Here we present a ligand-inducible labeling strategy for intracellular proteins based on fluorescent, 25-nm-sized, genetically encoded multimeric particles (GEMs).
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