Sarah Vreugde

Jun 4, 2024 | microbiologyresearch.org | George Bouras |Louise M Judd |Robert Edwards |Sarah Vreugde

Article metrics loading... /content/journal/mgen/10.1099/mgen.0.001254Download as PowerPoint /deliver/fulltext/mgen/10/6/mgen001254.html?itemId=/content/journal/mgen/10.1099/mgen.0.001254&mimeType=html&fmt=ahah References Delahaye C, Nicolas J. Sequencing DNA with nanopores: troubles and biases. PLoS One 2021; 16:e0257521 [View Article] [PubMed][Google Scholar] Lerminiaux N, Fakharuddin K, Mulvey MR, Mataseje L.

Mar 10, 2024 | biorxiv.org | George Bouras |Louise M Judd |Robert Edwards |Sarah Vreugde

AbstractIt is now possible to assemble near-perfect bacterial genomes using Oxford Nanopore Technologies (ONT) long reads, but short-read polishing is still required for perfection. However, the effect of short-read depth on polishing performance is not well understood. Here, we introduce Pypolca (with default and careful parameters) and Polypolish v0.6.0 (with a new careful parameter).