George Bouras

Jun 4, 2024 | microbiologyresearch.org | George Bouras |Louise M Judd |Robert Edwards |Sarah Vreugde

Article metrics loading... /content/journal/mgen/10.1099/mgen.0.001254Download as PowerPoint /deliver/fulltext/mgen/10/6/mgen001254.html?itemId=/content/journal/mgen/10.1099/mgen.0.001254&mimeType=html&fmt=ahah References Delahaye C, Nicolas J. Sequencing DNA with nanopores: troubles and biases. PLoS One 2021; 16:e0257521 [View Article] [PubMed][Google Scholar] Lerminiaux N, Fakharuddin K, Mulvey MR, Mataseje L.

Mar 10, 2024 | biorxiv.org | George Bouras |Louise M Judd |Robert Edwards |Sarah Vreugde

AbstractIt is now possible to assemble near-perfect bacterial genomes using Oxford Nanopore Technologies (ONT) long reads, but short-read polishing is still required for perfection. However, the effect of short-read depth on polishing performance is not well understood. Here, we introduce Pypolca (with default and careful parameters) and Polypolish v0.6.0 (with a new careful parameter).

Dec 19, 2023 | biorxiv.org | Ryan Cook |Andrea Telatin |George Bouras |Antonio Pedro Camargo

AbstractThe majority of bacteriophage diversity remains uncharacterised, and new intriguing mechanisms of their biology are being continually described. Members of some phage lineages, such as the Crassvirales, repurpose stop codons to encode an amino acid by using alternate genetic codes. Here, we investigated the prevalence of stop codon reassignment in phage genomes and subsequent impacts on functional annotation.