Snehajyoti Chatterjee

Oct 22, 2024 | biorxiv.org | Snehajyoti Chatterjee |Yann Vanrobaeys |Annie Gleason |Brian Park

AbstractDirect electrical stimulation has been used for decades as a gold standard clinical tool to map cognitive function in neurosurgery patients1-8. However, the molecular impact of electrical stimulation in the human brain is unknown.

Nov 4, 2023 | nature.com | Yann Vanrobaeys |Zeru Peterson |Snehajyoti Chatterjee |Li-Chun Lin |Thomas Nickl-Jockschat |Ted Abel | +2 more

Sleep deprivation has far-reaching consequences on the brain and behavior, impacting memory, attention, and metabolism. Previous research has focused on gene expression changes in individual brain regions, such as the hippocampus or cortex. Therefore, it is unclear how uniformly or heterogeneously sleep loss affects the brain. Here, we use spatial transcriptomics to define the impact of a brief period of sleep deprivation across the brain in male mice. We find that sleep deprivation induced pronounced differences in gene expression across the brain, with the greatest changes in the hippocampus, neocortex, hypothalamus, and thalamus. Both the differentially expressed genes and the direction of regulation differed markedly across regions. Importantly, we developed bioinformatic tools to register tissue sections and gene expression data into a common anatomical space, allowing a brain-wide comparison of gene expression patterns between samples. Our results suggest that distinct molecular mechanisms acting in discrete brain regions underlie the biological effects of sleep deprivation. Sleep deprivation impacts molecular changes across brain regions. Here, the authors utilize a spatial. transcriptomics approach to elucidate acute sleep deprivation-induced gene expression signature. across regions and subregions of the brain.