Stanislav S. Rubakhin

3 weeks ago | nature.com | Pei Su |Stanislav S. Rubakhin |Michael Caldwell |Jonathan V. Sweedler

AbstractWe perform intact proteoform profiling of 10,809 endogenous single cells from the rat hippocampus using single-cell proteoform imaging mass spectrometry (scPiMS). scPiMS directly extracts whole proteins and demonstrates high throughput for MS-based single-cell proteomics compared with existing approaches. We develop an informatics workflow dedicated to this datatype and use it to assign neurons, astrocytes or microglia cell types according to their proteoform signatures.

Feb 16, 2024 | nature.com | Yuxuan Xie |Daniel Castro |Stanislav S. Rubakhin |Jonathan V. Sweedler

AbstractSpatial omics technologies can reveal the molecular intricacy of the brain. While mass spectrometry imaging (MSI) provides spatial localization of compounds, comprehensive biochemical profiling at a brain-wide scale in three dimensions by MSI with single-cell resolution has not been achieved. We demonstrate complementary brain-wide and single-cell biochemical mapping using MEISTER, an integrative experimental and computational mass spectrometry (MS) framework.