Thomas Vanderstichele

Jul 24, 2024 | cell.com | Thomas Vanderstichele |Katie L. Burnham |Niek de Klein |Manuel Tardaguila

SummaryGene misexpression is the aberrant transcription of a gene in a context where it is usually inactive. Despite its known pathological consequences in specific rare diseases, we have a limited understanding of its wider prevalence and mechanisms in humans. To address this, we analyzed gene misexpression in 4,568 whole-blood bulk RNA sequencing samples from INTERVAL study blood donors.

Jan 23, 2024 | biorxiv.org | Jonas Koeppel |Raphael Ferreira |Thomas Vanderstichele |Lisa Maria Riedmayr

AbstractWhile protein-coding genes are characterized increasingly well, 99% of the human genome is non-coding and poorly understood. This gap is due to a lack of tools for engineering variants that affect sequence to the necessary extent. To bridge this gap, we have developed a toolbox to create deletions, inversions, translocations, and extrachromosomal circular DNA at scale by highly multiplexed insertion of recombinase recognition sites into repetitive sequences with CRISPR prime editing.

Nov 19, 2023 | biorxiv.org | Katie L. Burnham |Niek de Klein |Manuel Tardaguila |Thomas Vanderstichele

AbstractGene misexpression is the aberrant transcription of a gene in a context where it is usually inactive. Despite its known pathological consequences in specific rare diseases, we have a limited understanding of its wider prevalence and mechanisms in humans. To address this, we analyzed gene misexpression in 4,568 whole blood bulk RNA sequencing samples from INTERVAL study blood donors.