Lisa Maria Riedmayr

1 month ago | biorxiv.org | Ahmed Mahas |Raphael Ferreira |Lisa Maria Riedmayr |George M Church

AbstractProgrammable technologies that sense specific nucleic acid signatures in living cells and trigger cellular functions hold significant potential for biotechnology and medicine. Here, we developed SONAR (Sensing Of Nucleic acids using ASOs and Reverse-transcriptases), a platform that enables the detection of target DNA and RNA sequences and triggers controlled gene expression in human cells.

Jan 23, 2024 | biorxiv.org | Jonas Koeppel |Raphael Ferreira |Thomas Vanderstichele |Lisa Maria Riedmayr

AbstractWhile protein-coding genes are characterized increasingly well, 99% of the human genome is non-coding and poorly understood. This gap is due to a lack of tools for engineering variants that affect sequence to the necessary extent. To bridge this gap, we have developed a toolbox to create deletions, inversions, translocations, and extrachromosomal circular DNA at scale by highly multiplexed insertion of recombinase recognition sites into repetitive sequences with CRISPR prime editing.

Oct 18, 2023 | nature.com | Lisa Maria Riedmayr |Klara Sonnie Hinrichsmeyer |Michael Bartoschek |Christian Grimm |Jan Wijnholds |Stefanie Fenske | +1 more

AbstractLarge genes including several CRISPR-Cas modules like gene activators (CRISPRa) require dual adeno-associated viral (AAV) vectors for an efficient in vivo delivery and expression. Current dual AAV vector approaches have important limitations, e.g., low reconstitution efficiency, production of alien proteins, or low flexibility in split site selection. Here, we present a dual AAV vector technology based on reconstitution via mRNA trans-splicing (REVeRT).

Feb 7, 2023 | biorxiv.org | Lisa Maria Riedmayr |Klara Sonnie Hinrichsmeyer |Stefan Bernhard Thalhammer |Nina Karguth

Thank you for your interest in spreading the word about bioRxiv. NOTE: Your email address is requested solely to identify you as the sender of this article. Your Email * Your Name * Send To * Enter multiple addresses on separate lines or separate them with commas. Message Subject (Your Name) has forwarded a page to you from bioRxiv Message Body (Your Name) thought you would like to see this page from the bioRxiv website.