Tyler J. Lefevre

Aug 4, 2024 | nature.com | Kevin Knight |Brian Krumm |W. Grant Ludlam |Meng Cui |Tyler J. Lefevre |Wenyuan Wei | +9 more

AbstractMany neurotransmitter receptors activate G proteins through exchange of GDP for GTP. The intermediate nucleotide-free state has eluded characterization, due largely to its inherent instability. Here we characterize a G protein variant associated with a rare neurological disorder in humans. GαoK46E has a charge reversal that clashes with the phosphate groups of GDP and GTP.