Vitaliy Mykhaylyk

2 months ago | biorxiv.org | David Hollingworth |Karl F Herold |Geoff Kelly |Vitaliy Mykhaylyk

AbstractVoltage-gated sodium channels (VGSCs), key mediators of excitability and synaptic transmission, are established and functionally relevant targets for volatile anaesthetic (VA) action. Using the structurally homologous prokaryotic VGSCs NavMs and NaChBac as models, we present a structure-function analysis of VGSC-VA interactions.

Nov 4, 2024 | biorxiv.org | David Hollingworth |Karl F Herold |Geoff Kelly |Vitaliy Mykhaylyk

AbstractVolatile general anesthetics are used for inhalational anesthesia in hundreds of millions of surgical procedures annually, yet their mechanisms of action remain unclear. Membrane proteins involved in cell signaling are major targets for anesthetics, and voltage-gated ion channels that mediate neurotransmission, movement, and cognition are sensitive to volatile anesthetics (VAs).

Sep 28, 2024 | nature.com | Frances Thomas |Mohamed Fouda |Vitaliy Mykhaylyk |Martin B. Ulmschneider |Peter C. Ruben

AbstractNeuronal hyperexcitability is a key element of many neurodegenerative disorders including the motor neuron disease Amyotrophic Lateral Sclerosis (ALS), where it occurs associated with elevated late sodium current (INaL). INaL results from incomplete inactivation of voltage-gated sodium channels (VGSCs) after their opening and shapes physiological membrane excitability. However, dysfunctional increases can cause hyperexcitability-associated diseases.

Oct 12, 2023 | nature.com | Kamel El Omari |Vitaliy Mykhaylyk |Christian M. Orr |Maria Romano |Luca Vecchia |Miki Senda | +10 more

AbstractDespite recent advances in cryo-electron microscopy and artificial intelligence-based model predictions, a significant fraction of structure determinations by macromolecular crystallography still requires experimental phasing, usually by means of single-wavelength anomalous diffraction (SAD) techniques. Most synchrotron beamlines provide highly brilliant beams of X-rays of between 0.7 and 2 Å wavelength.