Xian Du

Apr 19, 2024 | genomebiology.biomedcentral.com | Xian Du |Chunyu Yang |Lin Dai |Siyu Zhou |Mengyang Wang |Shuang Wu | +9 more

HepG2 (HB-8065), HEK293T (CRL-11268), and derived cell lines were cultured according to ENCODE cell culture protocol. Briefly, HepG2 cells were cultured in MEM medium (Gibco, 11,095,080), supplemented with 10% fetal bovine serum (NEWZERUM, FBS-S500), 1 mM non-essential amino acids (Gibco, 11,140,050), 1 mM sodium pyruvate (Gibco, 11,360,070), 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco, 15,140,148).